Skip to main content

Parallel on-chip gene synthesis and application to optimization of protein expression..

By

Low-cost, high-throughput gene synthesis and precise control of protein expression are of critical importance to synthetic biology and biotechnology. Here we describe the development of an on-chip gene synthesis technology, which integrates on a single microchip the synthesis of DNA oligonucleotides using inkjet printing, isothermal oligonucleotide amplification and parallel gene assembly. Use of a mismatch-specific endonuclease for error correction results in an error rate of ~0.19 errors per kb. We applied this approach to synthesize pools of thousands of codon-usage variants of lacZα and 74 challenging Drosophila protein antigens, which were then screened for expression in Escherichia coli. In one round of synthesis and screening, we obtained DNA sequences that were expressed at a wide range of levels, from zero to almost 60% of the total cell protein mass. This technology may facilitate systematic investigation of the molecular mechanisms of protein translation and the design, construction and evolution of macromolecular machines, metabolic networks and synthetic cells.

source – Nature.

Comments

Post a Comment

Popular posts from this blog

Influenza A detection by MDCK cell line

By
The influenza A (IA) virus is the principal cause of the outbreaks of flu. A large number of laboratories participate in the worldwide surveillance of influenza virus activity and contribute to the early recognition of newly emerging epidemic strains. Differentiation between influenza A and B viruses and determination of the subtypes of influenza A virus isolates are the first steps in the characterization of influenza viruses. This analysis is traditionally done by hemagglutination inhibition (HI) tests with specific antisera raised in ferrets, chickens, or sheep. The diagnosis of Influenza A is largely clinical. Nevertheless, it is necessary to carry out some form of rapid antigenic diagnosis and the culture of respiratory samples to confirm the etiology of the respiratory disease and to determine the antigenic characteristics of the epidemic strains. Although the “gold standard” isolation technique is inoculation in embryonated hens’ eggs, the technical difficulties involved and t...
87 comments
Read more

ICMR Fellowships - Research Associate and Senior Research Fellow (RA and SRF)

By
The Indian Council of Medical Research awards the following categories of fellowships to young scientists to enable them to carry out research in the field of Biomedical Sciences at the permanent Institutes of the Council, other Biomedical Research Institutes, Medical Colleges, and Universities in India where adequate laboratories and other facilities to carry out research are available: 1. Research Associates 2. Senior Research Fellowships Fellowship Rules APPLICATIONS : Applications for the fellowships are invited by advertisement in newspapers and by the issue of a circular to all medical colleges, institutes etc. The following conditions are required to be fulfilled: The applications should be sent through the Guide and the Head of the Institute where the candidate proposes to work. If employed, a certificate from his employer to accept the fellowship should be submitted. The candidate should not draw any stipend or salary or be in receipt of any other type of fin...
16 comments
Read more

SOME INSTITUTE FOR SUMMER TRAINING/FINAL YEAR DISSERTATION/INTERNSHIP..

By
Center For Cellular and Molecular Biology (CCMB) <http://www.ccmb.res.in/>. Central Drug Research Institute (CDRI) <http://www.cdriindia.org/> Central Food Technological Research Institute (CFTRI) <http://www.cftri.com/> Central Institute of Medicinal and Aromatic Plants (CIMAP), <http://www.cimap.res.in/> Central Leather Research Institute (CLRI) Chennai <http://www.clri.org/> Institute of Genomics and Integrative Biology (IGIB)<http://www.igib.res.in/> Institute of Himalayan Bioresource Technology (IHBT),<http://www.ihbt.res.in/> Indian Institute of Chemical Biology (IICB)<http://www.iicb.res.in/> Indian Institute of Chemical Technology (IICT), <http://www.iictindia.org/> Institute of Microbial Technology (IMTECH)<http://imtech.res.in/> Indian Toxicology Research Center (ITRC) <http://www.itrcindia.org/> National Chemical Laboratory (NCL) Pune <http://www.ncl-india.org/> National Environmental Engineering Resear...
260 comments
Read more